6His protein

Henri M.H. Spronk henri.spronk at bioch.unimaas.nl
Wed Jan 28 11:49:04 EST 1998

After expression (in E.coli) of a recombinant human protein as part of a
6His-DHFR-fusion protein and purification using a Ni-NTA-column, the
protein is insolluble. The protein is soluble in a solution of pH 12, but
not at 7.5. Does anybody have experience with protein solubillization?

H.M.H. Spronk
Department of Biochemistry
University Maastricht
The Netherlands

henri.spronk at bioch.unimaas.nl

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