Protein purification using guanidinum hydrochloride

Randall C Willis willis at gandalf.psf.sickkids.on.ca
Wed Jan 21 09:43:31 EST 1998

James wrote:
I now need to refold the protein; does anyone have any tips on
temperature, rate of GuHCl dilution, etc., to optimise the formation of
soluble refolded monomer?


I would recommend that you check out some of the past posts by people on
the bionet archives
in the proteins and methods groups as this topic has come up a lot over
the last two years and I have made suggestions innumerable times.  Both
have search engines, so you may want to check out keywords like
guanidine, refolding or my name (for my previous posts).

I don't mean for this to sound like I'm slogging you off, but typing and
retyping the same information gets a bit wearisome.

Good luck, Randy

Randall C Willis, Researcher
Biochemistry Research, Hospital for Sick Children
3522-555 University Ave
Toronto, ON

416-813-5933 (ph)  -5022 (fax)
willis at gandalf.psf.sickkids.on.ca

Randall C Willis, Publisher
Aliquotes Press
Aliquotes: A Journal Of Molecular and Biochemical
              Humour and Information
58 Balfour Ave.
Toronto, ON

416-691-2921 (ph)  416-813-5022 (fax)
roger at xybercom.net

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