James wrote:
I now need to refold the protein; does anyone have any tips on
temperature, rate of GuHCl dilution, etc., to optimise the formation of
soluble refolded monomer?
James,
I would recommend that you check out some of the past posts by people on
the bionet archives
http://www.bio.net/archives.html
in the proteins and methods groups as this topic has come up a lot over
the last two years and I have made suggestions innumerable times. Both
have search engines, so you may want to check out keywords like
guanidine, refolding or my name (for my previous posts).
I don't mean for this to sound like I'm slogging you off, but typing and
retyping the same information gets a bit wearisome.
Good luck, Randy
Randall C Willis, Researcher
Biochemistry Research, Hospital for Sick Children
3522-555 University Ave
Toronto, ON
M5G 1X8 CANADA
416-813-5933 (ph) -5022 (fax)
willis at gandalf.psf.sickkids.on.ca
Randall C Willis, Publisher
Aliquotes Press
Aliquotes: A Journal Of Molecular and Biochemical
Humour and Information
58 Balfour Ave.
Toronto, ON
M4C 1T6 CANADA
416-691-2921 (ph) 416-813-5022 (fax)
roger at xybercom.net
