Sophie,
What did you use to determine the MWt of your protein? Is it
calculated? Determined by gel filtration? Glycosylation will affect the
mobility on SDS-PAGE. However, so will extremes of pI, shape, and a few
other things.
Bruce
Bruce G. Allen, Ph.D. Phone: 514-376-3330 (3591)
Institut de Cardiologie de Montreal FAX: 514-376-1355
Centre de Recherche EMAIL: allen at icm.umontreal.ca
5000, rue Belanger est
Montreal, Quebec
Canada
H1T 1C8
