Cornelius Krasel wrote:
>> Markus Mentzer <mmentzer at students.wisc.edu> wrote:
> > I've been trying to find methods of ligating strands of proteins to
> > one another. Specifically, I have been trying to find ways to
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Part of reply deleted.
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>> You might also want to look into ubiquitin ligases which conjugate (fairly
> unspecific) the small protein ubiquitin to epsilon amino groups of
> lysines.
>> --Cornelius.
Cornelius is right in that you could use the ubiquitin ligases to ligate
two proteins together. Our lab has found a way to direct to attachment
of ubiquitin to preselected proteins (see PNAS 92:9117). (The exact site
of ubiquitin attachment on the targeted protein is non-specific.) It is
also possible to make rather large fusions to the C-terminus of
ubiquitin and still have it be ative in this pathway. If you just want
to bring two proteins into proximity you might be able to use an
approach like this.
Mark
