Zn precipitation of proteins

Kurt Thorn thorn at itsa.ucsf.edu
Thu Jan 15 19:33:41 EST 1998

In article <34BE4ACD.611E at orac.sund.ac.uk>,
gerald  <hs0lbe at orac.sund.ac.uk> wrote:
>Pete wrote:
>> Does anyone know any specific protein complexes that are precipitated
>> from tissue homogenates by the addition of 1 mM Zn chloride.
>> I get a huge precipitate when I add more than 0.1 mM Zn chloride that
>> seems to contain specific proteins. If I only had a clue what these
>> proteins could be...

Just a wild guess, but could it be tubulin?  I know tubulin forms zinc
sheets at around that zinc concentration, and that they will precipitate
rather nicely.


      Kurt Thorn - thorn at itsa.ucsf.edu - http://motorhead.ucsf.edu/~thorn
"The universe we observe has precisely the properties we should expect if
there is at bottom not design, no purpose, no evil and no good, nothing but
pointless indifference."  -- Richard Dawkins

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