IUBio

Zn precipitation of proteins

Pete pkursula at cc.oulu.fi
Thu Jan 15 01:49:47 EST 1998


On Wed, 14 Jan 1998, Nicole Rapior wrote:

> Pete wrote:
> > 
> > Does anyone know any specific protein complexes that are precipitated
> > from tissue homogenates by the addition of 1 mM Zn chloride.
> > 
> > I get a huge precipitate when I add more than 0.1 mM Zn chloride that
> > seems to contain specific proteins. If I only had a clue what these
> > proteins could be...
> > 
> 
> Hi Pete,
> 
> as you didn't mentioned any other conditions, is it possible that
> you use a phosphate-buffer? I don't want to insult, you but a lot of
> people forget that zinc phosphate isn't very soluable! It might be the
> reason for your precipitate.
> 
> Bye   Nicole
> 

They are proteins, very specific ones. This HAS been checked by SDS PAGE.
They precipitate when Zn is increased. No phosphate is present, but other
conditions I won't reveal :) No there's nothing special there, and it
happens in different buffer systems, too.




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