Hi Jennifer,
You may not have the same bacteria that you started
with. Do they smell normal? It is quite easy to tansform
other contaminating bacteria; I've done it my self in grad.
student days. That would explain the funny color and the
less than optimal expression. Try some fresh bacterial
stocks. All the best
> Hi! My name is Jennifer Morrison. I am a MSc student here at UPEI, and
> I was wondering if any of you could help me with a problem I've been
> experiencing. In our lab, we have been expressing a de novo protein in
> bacterial cells and have had no problems in the past. Lately, our cells
> have been gray-black in color when harvested and the protein hasn't
> shown up very well, if at all, on gel. If you have any suggestions as
> to what our problem may be, or what we could do to fix this problem,
> please e-mail me at jmorrison at upei.ca>> Thank you very much!
>>
_________________________________________________________
John Marcus Marcus at tpp.uq.edu.au (Dr J.Marcus)
Cooperative Research Centre for Tropical Plant Pathology
5th Level John Hines Building
University of Queensland
St. Lucia, QLD 4072
AUSTRALIA
Fax: 61-7-3365-4771
Phone: 61-7-3365-4764
