problems during protein expression

Marcus, Dr J. J.Marcus at BOTANY.UQ.EDU.AU
Thu Jan 15 01:52:54 EST 1998

Hi Jennifer,
     You may not have the same bacteria that you started 
with.  Do they smell normal?  It is quite easy to tansform 
other contaminating bacteria; I've done it my self in grad. 
student days.  That would explain the funny color and the 
less than optimal expression.  Try some fresh bacterial 
stocks.  All the best

> Hi!  My name is Jennifer Morrison.  I am a MSc student here at UPEI, and
> I was wondering if any of you could help me with a problem I've been
> experiencing.  In our lab, we have been expressing a de novo protein in
> bacterial cells and have had no problems in the past.  Lately, our cells
> have been gray-black in color when harvested and the protein hasn't
> shown up very well, if at all, on gel.  If you have any suggestions as
> to what our problem may be, or what we could do to fix this problem,
> please e-mail me at jmorrison at upei.ca
> Thank you very much!

John Marcus            Marcus at tpp.uq.edu.au (Dr J.Marcus)
Cooperative Research Centre for Tropical Plant Pathology
5th Level John Hines Building
University of Queensland
St. Lucia, QLD 4072

Fax: 61-7-3365-4771
Phone: 61-7-3365-4764

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