Hi, Netters.
I am trying to express the cpp32(caspase-3) in yeast nucleus.
The protease(caspase-3) expressed was designed to contain SV40
nuclear localize signal in its N terminal.
The problem is
1. The protease is activated in the yeast by proteolysis of the middle
of the protein. Although the cleaved fragment is not dissociated(actually
interacts with other fragments to become a complex), I wonder if the SV40
nuclear localize signal(fused in the N-terminal) is working.
2. Caspase-3 is known to exist in cytosol and nucleus. Is it possible to
make this protease exist only in nucleus?
Any information or advice regarding above problems will be greatly appreciated.
Thanks in advance.
Bye.
--
___________________________________________________________________________
KAIST, Daejon Korea. Dooyeon Kim ......................
dykim at bioneer.kaist.ac.kr
___________________________________________________________________________
--
___________________________________________________________________________
KAIST, Daejon Korea. Dooyeon Kim ......................
dykim at bioneer.kaist.ac.kr
___________________________________________________________________________
