In article <34B5988F.41C6 at interzon.com>, Uncle S <UncleS at interzon.com> wrote:
>Lion wrote in this pop quiz:
>>>> How many methods are available for assaying protein-protein interaction?
>>Just two, I'm afraid.
>>> What are these methods?
>>Well, there's the yeast two hybrid (Tm) and the other one doesn't
>matter.
Well,
there are a whole host of quantitative methods as well. I don't off hand
know of a good reference but there may be one out there. I will just list
a few for you here.
analytical ultracentrifugation - perhaps the best, both velocity and
equilibrium
BIACore and other plama resonsance techniques - quantitative? well you be
the judge :)
calorimetry
hydrodynamic techniques - column chromatography, large zone and traditional
light scattering
spectroscopic techniques - CD,fluorescene etc....changes in signal with
protein concentration
affintity chromatography
equilibrium dialysis - if one protein/ligand is small enough
mass spectroscopy
this is a quick list, i can probably think of half a dozen other
techniques. If you are interested in a particular system, feel free to
drop me some mail.
Peter Prevelige
Associate Professor, Dept. of Microbiology
Univ. of Alabama @ Birmingham
