IUBio

ScFv - help!

jamie bickley jfb at dl.ac.uk
Thu Jan 8 09:49:54 EST 1998


Hi!

I'm having afew problems whilst attempting to purify a ScFv sample.
The main problem is, it appears to be binding to the column!
I have tried and Ion exchange column and a simple exchange
chromatography column.  I have also tried varying the pH of the
buffers.  
Any idea's?

I would appreciate any help.

Thanks in advance

Joanne Burns
J.C.Burns at dl.ac.uk



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