I am attempting to express a phage protein in E. coli that appears to be
quite toxic. I am able to transform JM109 just fine with my pET vector
construct but am unable to transform any strain containing T7 RNA
polymerase (BL21 DE3). I have heard of people infecting JM109 with
recombinant phage containing T7 polymerase. Does anyone know of a source
for these phage (lambda or M13)?
I have also read about a group (Appl Microbiol Biotechnol 47:241-245.)
using another plasmid containing a T7 promoter to sop up leaky T7
polymerase in the cell. Does anyone know of a source for this plasmid?
Please reply by e-mail to pietz at oncology.wisc.edu
--
Vladimir Svetlov
McArdle Lab for Cancer Research
Dept. Oncology
UW-Madison
1400 University Ave.
Madison, WI 53706
