Hi all
We currently have a protein solution which is 99% (plus) pure,
however we have discovered protease activity within our sample.
This protease activity can be prevented by addition of EDTA
(metallo-protease?),
and using several protease inhibitors.
The question I have, is how can I discover if the protein we are working
on
has intrinsic protease activity (it is not supposed to) or if the less
than
1% impurity is infact a protease ?
We have tried silver stained gels
Isoelectric focusing
SDS gels
and Native gels to see if we can detect the contaminant.
Any advice or help would be greatly appreciated
Alan Riboldi-Tunnicliffe
atunn at imb-jena.de
