POROS columns seem to have poor binding capacity anyway in my opinion
for IMAC. EDTA and Tris are likely to further reduce binding. Also,
what is your flow rate for binding?
--
Brian M Burkhart, Ph.D.
Hauptman-Woodward Medical Research Institute
73 High Street Buffalo, NY 14203
Phone: (716)856-9600 x313 FAX: (716)852-6086
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://iubio.bio.indiana.edu/bionet/mm/proteins/attachments/19980821/7a095090/attachment.html
