Does anyone know of a way to optimize thrombin cleavage of GST fusion such
that only the five base site is cleaved and not the three base sites. I
am getting the majority of cleavage at the five base site but also some
annoying cleavage at the three base sites present in my protein. I have
heard of using heparin in the cleavage reaction, does anyone know of this?
Any help would be appreciated.
Mike
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Michael DiDonato
Structural Biology and Biochemistry
The Hospital for Sick Children
Toronto, Ontario, Canada
