In article <35D1D215.CC2EB5C9 at bcm.tmc.edu>, ah692010 at bcm.tmc.edu wrote:
:Hi!
:Could anyone please suggest the best way to show an interaction between
:proteins that transiently interact? Any suggestions would be terrific.
:Thank you.
What exactly do you mean? In every solution, every molecule transiently
interacts with another molecule (they hit each other and then have higher
or lower chances of staying together for some time).
Do you mean that the association constant is so low so that
half-time of the complex is very short?
Or you mean that there is some high affinity interaction which
is disturbed by a third party in a timely fashion?
The approaches, I think, would be very different. Also, it is
important to know what system you have - in vitro or in vivo,
whether you are dealing with pure proteins available in large
quantities or not, etc. There are so amny ways to study
interactions between molecules that the answer to your question
becomes impossible without knowing what's given.
- Dima
