S-35 Methionine labelling yeast cells?

Dr Engelbert Buxbaum eb15 at le.ac.uk
Mon Aug 10 16:33:44 EST 1998

Adrian Bracken wrote:
> Hi,
> I am wondering if it is possible to S-35 Methionine label yeast cells?

You can label proteins in any actively metabolizing organism by
incubatin in a medium without "cold" Met (or Cys) by adding the labeled
amino acid (there are mixtures of 35-S Met and Cys available which are
cheaper and offer better labeling than either aa alone). Incubate the
cells for an appropriate amount of time (try a time series with a small
amount first, say 10 min to 2-3 h). The correct time depends on the sort
of protein (synthesis and turnover rates, posttranslational processing

Avoid complex mixtures of aa like pepton, yeast extract, serum ect in
the medium as they would dilute the radioactivity.

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