> Hi,
>> Recently, I was involved in doing western blot on retina of SD rat and
> I
> found that there was a lot sticky, jelly like structure after boiling
> with TBS with SDS, triton x-100 buffer although I centrifuged it can
> sonicate the supernatant, it still here. How should I do so that I can
>> get rid of it? These sticky material gave vertically line rather than
> horizontal band in SDS page or NC membrane.
>> Thank you!