In article <i.mcfarlane-2807981850060001 at mac017044.guys.icnet.uk>, i.mcfarlane at icrf.icnet.uk (Ian McFarlane) wrote:
>Once upon a time somebody mentioned that there was some trick with
>dialysis tubing and vaccuum that could be performed to concentrate
>proteins (flash dialysis?). Does anyone know how to do this or is my
>memory failing me?
>>Thanks,
>>Ian Mc
It's been a while since I did it but as I recall my vacuum dialysis set was
as follows:
Use an empty vacuum flask and rubber stopper with two large holes. Put one
P1000 pipette tip (with end melted) in one hole in the stopper as a plug. You
will want to use this to release the vacuum when you are finished. Fill the
dialysis tubing with your sample leaving one end open. Slip it up through
the second hole in the stopper. Now use the other pipette tip (P1000) with its
tapered end cut off and slip it in open end of the tubing and press it tight
in the stopper hole. The dialysis tubing should be in the flask and now you
apply vacuum using a water aspirator. Buffer will be drawn out of the
membrane and into the flask. You can continue to add more sample to the
dialysis membrane through the pipette tip.
Mark Lamkin
