Has anyone had any problems Western blotting onto PVDF in CAPS buffer? I am
trying to get a sequence on my protein and it appears that the efficiency
of transfer is letting me down. Could it be the buffer, (10mM CAPS, 10%
methanol, pH 11) ? I have had no problems blotting onto nitrocellulose
using tris-glycine buffers.
Thanks for your time
Karen Spink
