It depends which ones you want to break. At least in rabbit IgG the interH
chain -SS- bond can be cleaved with about 0.1-0.2 mM DTT at pH 8, while the
interHL bonds need about 1 mM DTT. In the absence of denaturants, the
intra-domain bonds are relatively resistant.
In article <mbzrl-290997145303 at pmbfjd0.nottingham.ac.uk>,
mbzrl at mbn1.biochem.nottingham.ac.uk (Rob) wrote:
|| Hi
|| Can anyone suggest the concentration of DTT (or mercaptoethanol) and
|| conditions which would be needed to break the disulphides in an antibody.
|| Thanks
|| Rob
Simon
--
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Dr Simon B Easterbrook-Smith Voice: (+) 612 9351 3905
Department of Biochemistry FAX: (+) 612 9351 4726
University of Sydney Email: sbe at biochem.usyd.edu.au
Sydney NSW 2006
AUSTRALIA
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