MAybe the positively charged Zn ions just travel in the other direction?
Do you have Zn ions in the electrophoresis buffer? It seems that the
protein needs Zn to stay together. You know, an electric field makes
everything with an electric charge... move. I would add Zn to the gel and
to the running buffer.
Just my guess
On 27 Sep 1997, Oliver May wrote:
> Hi, all
>> I have purified a zinc containing enzyme which dissociates during natif
> PAGE into its subunits. (Buffer and pH is not the reason for dissociation)
> The enzyme also dissociates if it is incubated with chelators like EDTA,
> which was analysed by gelfitration.
>> Here my question:
> Are there any reports on dissociation of metal dependent proteins during
> PAGE, or does anybody have an idea why the protein dissociates?