Hi everyone,
Does anyone know the true detection limits of silver staining? The
red-book describes 3 methods of silver staining: a long ammoniacal
protocol, a shorter non-ammoniacal method, and a rapid 1 hour method. The
book claims that the first method can detect 2-5 ng of protein, though i
am a little skeptical. I have only tried the rapid method, and am lucky
to be able to detect 40-50 ng of protein after over-developing. So if
anyone has tried either of the lengthier methods, I would appreciate your
comments on how much you can detect. However, I understand that this
method is somewhat protein-dependent, and certain proteins just never
stain well.
thanks!
donny wong
wong5 at fas.harvard.edu
