I forgot to mention in my post yesterday that I am using Tricine gels.
>>I am analyzing a 3.5 kD protein (pI=9.3) by SDS-PAGE and am having problems
>visualizing it. This could be due either to poor staining or to diffusion
>of the protein out of the gel. Staining by Coomassie blue and silver stain
>are both poor, which makes me think diffusion is the problem. I have tried
>fixing the gel with 12% TCA/3.5% sulfosalicylic acid without success. I may
>try using glutaraldehyde next, but would like any advice from others who
>have had this problem.