Damn this server....somebody wrote:
: How do I concentrate a low molecular weight protein (less than 5kDa)
: from gel filtration fractions ie. dilute???, keeping in mind that
: filtration devices and dialysis tubing concentrate above this size.
One option which I've used on a limited basis (and have never taken the
time to quantify the results) is to swell a powdered gel filtration
medium--in your case Sephadex G10 or G15--in your pooled fractions. The
beads take up the water and, in general, exclude your protein if you
choose your matrix wisely. Good luck.
Steve Dahl
