IUBio

concentration of low molecular weight protein

Dr E. Buxbaum EB15 at le.ac.uk
Fri Sep 19 10:55:20 EST 1997


Alison Leakey wrote:
> 
> How do I concentrate a low molecular weight protein (less than 5kDa)
> from gel filtration fractions ie. dilute???, keeping in mind that
> filtration devices and dialysis tubing concentrate above this size.

Have you tried concentrating chromatographic techniques (reverse phase
chromatography, ion exchange)?



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