In article <3422A068.852 at le.ac.uk>, "Dr E. Buxbaum" <EB15 at le.ac.uk> wrote:
> Alison Leakey wrote:
> >
> > How do I concentrate a low molecular weight protein (less than 5kDa)
> > from gel filtration fractions ie. dilute???, keeping in mind that
> > filtration devices and dialysis tubing concentrate above this size.
>> Have you tried concentrating chromatographic techniques (reverse phase
> chromatography, ion exchange)?
You could run your gel filtration column in a volatile buffer such as
ammonium acetate and then freeze-dry (lyophilise). Usually with small
proteins/peptides RP-HPLC is the final step as this gives the protein in a
small volume of volatile buffer.
Ian Mc
