IUBio

concentration of low molecular weight protein

Alison Leakey leakeya at citec.qld.gov.au
Fri Sep 19 01:44:11 EST 1997


How do I concentrate a low molecular weight protein (less than 5kDa)
from gel filtration fractions ie. dilute???, keeping in mind that
filtration devices and dialysis tubing concentrate above this size.

I have tried ammonium sulphate precipitation (up to 80%) with poor
results. It just doesn't want to precipitate!!!

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Alison Leakey
PhD Student
North Queensland Clinical School Laboratory
Ground Floor, Pathology Building,
Townsville General Hospital
Phone me at (077) 81 9662
FAX me at (077) 81 9649
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