cleavage of protein in SDS-gel

[CRC Institute for Cancer Studies]

Hi, 

I am looking for a method to cleave a protein (ca. 400 kDa) in a gel. Due
to the size, I thought of BrCN. However, all methods I could get hold of
involved electroelution (which I think won't work with such a protein).
Maybe AspN or ArgC would be alternatives?

Thanks in advance,

Peter


p.weber at bham.ac.uk