Hello. I need help. I am working with leguminosae glycoprotein
(Lupinus albus, Phaseolus vulgaris, Vicia faba, ...). I want to
deglycosylate glycoproteins mantaining intact the peptidic chain so
that I can produce specific antybodies.
I've been reading some articles about this subject but so far I only
could found methods that preserve the sugar, breaking the peptide
chain. I am not interested in enzymatic release of O- and N-linked
oligosaccharide chains or release of N-linked chains by
hydrazinolysis.
Could someone help me.
TIA.
Astride
e-mail:aa13082 at isa.utl.pt
