I am trying to get some information about producting antibodies to GST
fusion proteins. I have two proteins I want to send off for
immunizations, but I need advice first:
1. To cleave or not to cleave? My fused piece is about 9 kD, and seems
pretty stable, but is it better to send the whole protein, which should
be more immunogenic?
2. Any recommendations on companies? My lab has used Cocalico before, but
I'm open to suggestions.
If you have done this, please send me your thoughts on the matter... I
have heard lots of conflicting things from many people!
Thanks in advance,
Mara Casar
(reply to casar at niehs.nih.gov or to the above address)
