On 1 Sep 1997, RSjolund wrote:
> I've got a 32 kDa protein from a plant cell plasma membrane. It is
> extracted well in a medium containing CHAPS, but if I precipitate it with
> ammonium sulphate (40% saturation) if does NOT go back into solution.
> What are some good techniques for concentrating the protein (prior to 2D
> gels). The goal is to sequence the protein.
>> Dick Sjolund
> Biological Sciences
> Univ. of Iowa
>>r-sjolund at uiowa.edu>>you can try TCA precipitation plus washing the pellet with cold (-20)
aceton. In order to ressuspend it put some non-ionic detergent in the
buffer.
Hope this helps!
