Hi,
I am trying to characterize a novel protease secreted by a bacterium.
Could anyone help me with the following questions please:
(i) What kind of tests must I do to characterize the type of protease by
its cleavage site? i.e. serine protease etc.
(ii) Do you know of any "general" p-nitrophenol protease substrates that
I can use to biochemically characterise the protease? (temperature, pH,
salt dependence etc). If not, what about any "specific" substrates for
other proteases that I could try?
Thanks for any help you can give.
Please reply by e-mail if poss.
Cheers,
Mark
_________________________________
Dr. Mark A. Blight,
CNRS URA2225,
Institut de Génétique et Microbiologie,
Université de Paris Sud,
Bâtiment 409,
91405 Orsay,
France.
Tel: +33 1 69156699
Fax: +33 1 69157808
e-mail: blight at igmors.u-psud.fr
Internet: http://www.igmors.u-psud.fr/holland/holland-home-page-gb.html
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