I've spent a lot of time trying to get a good Western blot of HIV-1 gp41
TM protein (from lysates of cos-7 cells transfected with a non-infectious
[pol-,pro-] proviral construct,IP'd with human anti-HIV serum) and am
unable to reproducibly get a satisfactory Western. This is because of the
sporadic appearance of a non-specific band that co-migrates with gp41. I
have tried 2 commercial monoclonal antibodies, ABi and NEN, both of which
are supposedly good for Western. Does anybody have any suggestions for me.
