In article <63r37p$cgv at ccshst05.cs.uoguelph.ca>,
dvadnais at uoguelph.ca (D. Vadnais) wrote:
>> I am going to use IEF slab gels from Bio-Rad to separate invertase
> isozymes from alfalfa. I have never done this procedure before, and I am
> not really sure of the protocol. I have only the protocols that are used
> for 2D gel electrophoresis using the capillary tubes. I need to know
> what buffers I should be using to run these gels, and what type of
> loading buffer I require for my samples.
> Thanks in advance for any information you can give me.
> Dave
I suggest you contact our offices at Pharmacia Biotech to get a free copy
of the Hoefer Protein Electrophoresis Applications Guide. It is complete
with theory, practice, pictures, illustrations, etc. Isoelectric focusing
is covered in extensive detail. Contact: Pharmacia Biotech Inc. 500,
Morgan Boulevard Baie d'Urfe Quebec H9X 3V1
514-457-7000
Ask for Marie-France Belanger, the electrophoresis specialist in your
area.
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