I am going to use IEF slab gels from Bio-Rad to separate invertase
isozymes from alfalfa. I have never done this procedure before, and I am
not really sure of the protocol. I have only the protocols that are used
for 2D gel electrophoresis using the capillary tubes. I need to know
what buffers I should be using to run these gels, and what type of
loading buffer I require for my samples.
Thanks in advance for any information you can give me.
Dave
