In article <01bce8a7$e01cfd70$373412ac at dough>, "Doug Hubatsch"
<Doug.Hubatsch at arcm.ca.astra.com> wrote:
> Anybody else come across this? When I sonicate bacteria in .1 mM PMSF/10
> mM EDTA, add 1% SDS and incubate for 30 min. at RT I get a DNA-like
> precipitate. I don't think its because of the SDS, boiling the mixture
> doesn't put the precipitate back into solution. The concentrations for
> PMSF and EDTA are well below other reported concentrations so I don't
> really have any idea what could be causing it.
>> Any ideas?
Is there any reason to think that this precipitate is something
other than DNA? It's been a while since I lysed bacteria, but
I remember the early steps of the process being quite gelatinous
in consistancy, not clearing up until after DNase treatment.
--
Eric Roush When an eel
edroush at acpub.duke.edu bites your heel,
also coache at aol.com makes you squeal,
pain you feel,
that's a moray.
