Lloyd Graham (lloyd.graham at molsci.csiro.au) wrote:
: I'm looking for any precedents where the domains of a monofunctional
: enzyme have been expressed separately as inactive proteins, but when mixed
: together they associate to form a complex with significant enzymatic
: activity.
Markby et. al., Science 262 p1895 (1993), separately expressed 2 halves of
a heterotrimeric G protein alpha chain; GTPase activity was regained
upon mixing the two domains.
Dennis
