I think the degrade of the marker is the answer.Try a new marker.
Ying Liu
On 31 Oct 1997, Obaid Yusuf Khan wrote:
> I am using the Biorad Kaleidoscope Prestained Standards to size my proteins
> on a 12% PAGE gel ("29:1). The markers below 31800 KD are very faint and
> fuzzy and I am unable to see the 17800 and 7100 KD markers.
> I am using the markers without any heat denaturing (I presume they dont require
> it).
> Does any one have any tips to offer me on this please.
> Thanks
> Obaid Khan
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