Dr Ron Croy (r.r.d.croy at durham.ac.uk) wrote:
: We need to use a sensitive protein stain to detect protein spots on 2D
: gels with the intention of sequencing these from PVDF blots (automated
: ABI 477A). Coomassie blue stains are out because they tend to interfere
: with the sequencing and clog up the reaction vessel. Silver staining
: methods are out due to the protein modification. We have tried a small
: sample of a stain called BT blu (originally from BT Scientific
: Technologies, Carlsbad, Ca) with promising results but cannot contact
: this company for further supplies (it has been suggested we contact
: Zolon or Zolan Research for a stain called 'Fast Stain'). Any comments
: and further contacts would be appreciated.
:: RRDC
If you are interested in staining the gel itself, rather than the
blot, you can elute protein and coomassie blue from the chunk of
excised gel, dry the eluted protein and stain, then remove the stain.
It's tedious, but possible.
The problem is that you are left with a thin film of protein dried
on the
walls of a small tube; it may not be suitable for your machine from a
sample-handling point of view. We used to use this for amino acid
analysis (not sequence) using the Millipore/Waters picotag system.
BTW, I think the company you refered to is Zoion, not Zolon.
Good luck with it.
Steve Driska, Physiology Department, Temple University Medical School
Philadelphia, PA 19140 USA (215) 707-3283
driska at astro.ocis.temple.edu
