Karl Fischer wrote:
>> In article <5gv4f2$9rp$1 at cnn.ksu.ksu.edu>, scotbean at ksu.ksu.edu (Scott
> Bean) wrote:
>> > I'm looking for references dealing with the CMC of SDS and the effects that
> > buffer salts might have on the CMC. Can anyone give some ref's to start with?
> > Is there any general rules concerning the CMC of detergents and ionic
> strength
> > of a buffer? (e.g Does the CMC always decrease if you increase ionic
> strenth).
>> Hi Scott,
>> There is a table on page 689 of "Antibodies: a laboratory manual" which
> has properties of many commonly used detergents including SDS. The CMC
> that is listed for SDS is 0.24 grams/100ml and the micelle molecular
> weight is 18,000. Hope this gives you a starting point for your quest.
>> Cheers
>> Karl the hepB guy
>> --
> Karl Fischer
>tyr-2 at bones.biochem.ualberta.ca
Hi there,
good starting points are the detergent manuals of either Boehringer
Mannheim or Calbiochem. Another one is a review written by Ari
Helenius and Kay Simons, Biochimica et Biophysica Acta. 415(1):29-79,
1975 Mar 25.
For ionic detergents the cmc - to my knolwedge - always goes down when
you increase the ionic strength, the cmc of non ionic detergents on
the other hand are not that much affected by the salt concentration.
I hope this helps
Thomas
