I've noticed that Perkin is charging about double for its "AmpliTaq"
relative to either Promega or Gibco (0.62US$/unit vs. 0.3/U). :) I
obtained a Promega Taq from our on-site freezer program recently "dead
in the water" (based on side by side comparisons).
I've seen the Biotechniques article from 95 wherein the authors describe
a primer pair for isolation of the polymerase gene from T. aquaticus,
the sublconing into coli under an overexression promoter, and the
purification of Taq in a single step from boiled coli lysate.
Anyone have an opinion on where to buy Taq? I'm quite interested that it
be free of any ribosomal sequence contaminants, as I was aware of that
being a problem for a colleauge working with environmental samples (who
subsequently prepared his own "cleaner" Taq) :)
Anyone try a single-step boil prep of there own for less sensitive work?
Please E-mail me copies of any responses directly,
Thanks very much,
Jim
J. Graham PhD
Wahsington University of St. Louis
