IUBio

Need Generic Method for Ptn Kinase Purification

Michael Myers myersm at ROCKVAX.ROCKEFELLER.EDU
Sun Mar 23 23:02:42 EST 1997


Let's say you wanted to purify as many different kinases as possible from a=
 tissue or cell line. Are there any affinity steps that would give a=
 reasonable degree of enrichment? For instance, could one use an ATP-coupled=
 resin? I am not interested in getting enzymatically active enzyme. Perhaps=
 there is a short list of successive affinity steps that would represent the=
 best compromises between selectivity, generality, and practicality.

TIA,

Mike





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