In article <5g20eq$dlo at clarknet.clark.net>, Harvard Morehead
<harvard at mail.clark.net> wrote:
> > *Urgent!*
> >
> > Some or probably quite many air bubbles seem to be trapped in my HPLC
> column: 18C reverse phase column, pore size 7um.
> > Could you give me any suggestions to remedy this problem as quickly as
> possible?
> > I am much disappointed not to be able to proceed my experiments any more.
> > :-(
I usually find that methanol or acetonitrile will dislodge gas trapped in
the column, I think what you should be asking is where this gas has come
from - check that you do not have a leak on the inlet side of your column
and thus that you are not actually introduciong air onto the column as you
run it.
