John Piper wrote:
>> Greetings everyone,
> PLEASE, someone help me regain my sanity!!! I am in the process of trying
> to sequence an N-terminal blocked protein, so obviously I am performing a
> series of digests. Well, to make a long story short, I am using the known
> sequence of the mouse isoform of this protein to make digestion
> predictions. I ran the mouse sequence through GCG's PeptideMap program,
> and one promising digest is ProEn, described in the proenz.dat file as
> Proline Endopeptidase. I have been trying, VERY unsuccessfully, to find a
> source of this enzyme. I even contacted GCG's tech support line, and they
> do not have a current source listed for this enzyme. From the information
> I have gathered, this is not an efficient (or popular) enzyme, which may
> explain its scarcity. Most of the references I have found are either from
> the early 80's or late 70's. If anyone knows of a source of Proline
> Endopeptidase, I would be eternally grateful if you would share that
> information with me. Thanks in advance for your replies.
>> John
Dear John
some years ago I ordered this enzyme from Seikagaku Corp. In the 1994
catalog the distributor of their products in the USA was:
Seikagaku America, INc.
30 West Gude Drive, Siute 260
Rockville, MD 20850-1161
Ph: 301-424-0546 or 1-800-237-4512
fax: 301-424-6961
I hope that this address is still o.k.
Many regards
Peter
--
Dr. Peter Hunziker
Universitaet Zuerich, Biochemisches Institut
Winterthurerstr. 190
CH-8057 Zuerich, Switzerland
Tel: 01/257 55 20
Fax: 01/363 79 47
E-mail: phunzi at bioc.unizh.ch
