Hi all,
I need to isolate different isoenzymes, which are of similar isoelectric
point. I tried to do it by preparative isoelectric focusing, but I can't
get satisfactory results suitable for crystallographic studies.
I know of two products that might interest me: chromatofocusing columns
and Isoprime (Multi-chambered Electrofocusing unit), both from
Pharmacia.
Any experience report with those or even other preparative isoelectric
focusing techniques will be appreciated.
Thank you in andvance
Matteo
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Matteo Antorini
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