hbkg at hitel.kol.co.kr wrote:
: We tried to dissolve DTNB, CAT substrate, in Tris buffer, and
: found that DTNB was not dissolved in Tris buffer at all, which
: was very strange. We changed water in vain.
: We found at last the mistake of using 1mM Tris instead of appropriate
: concentration.
: I am curious HOW TRIS AFFECTS DTNB SOLUBILITY.
What is the pH of the solution? 1 mM is not a very high concen-
tration and may not hold the pH to a pre-set value if you add a significant
amount of a solute that may be acidic or basic.
Good luck, someone should be able to help you in this group.
Steve
--
Steve Driska, Physiology Department, Temple University Medical School
Philadelphia, PA 19140 USA (215) 707-3283
driska at astro.ocis.temple.edu
