XiaoYin Zhong <medm049 at uabdpo.dpo.uab.edu> wrote:
>Dear Dr. protein:
>>I cloned a protein with His-tag at C-end. After I purified this perotein
>by Ni-column, SDS-PAGE gel show that the protein was degraded.What is
>the reason? Is there any other methods which can solve this problem
>except using proteinase inhibiter coke-tail or EDTA in the buffer?
>>Thank you for your help!
>>>>>qiu at orion.cmc.uab.edu
Are you working at 4 C ?
