>>> *Urgent!*
>> Some or probably quite many air bubbles seem to be trapped in my HPLC
column: 18C reverse phase column, pore size 7um.
> Could you give me any suggestions to remedy this problem as quickly as
possible?
> I am much disappointed not to be able to proceed my experiments any more.
> :-(
>> Thanks...
>>>> --
> email : newera at plaza.snu.ac.kr> address :
> Lee, Ji Hyun
> Laboratory of Physical Pharmacy(Prof. Lee, Bong Jin)
>> Seoul National University
> College of Pharmacy
> Shinlim-Dong, Kwanak-Gu
> Seoul 151-742, Korea.
>I have had some luck by running overnight at a slow rate (0.1 ml/min for an
analytical column), with the column with the outlet up, so that any bubbles
will float trhough the column, and using very thouroughly degassed water.
The water will dissolve any air, and any bubbles will be pushed up through
the column.
Good luck!
-Harvard Morehead
harvardm at aol.com
-no cool .sig
.
