pxpst2 at vms.cis.pitt.edu (THE GREEK) wrote:
>I am interested in getting some references to some precipitation methods
>other than TCA. The method must be suitable for low protein
>concentrations.
The most common method is probably the one described by Wessel and Fluegge in Anal. Biochem.,
somewhere in the early 80's, which uses chloroform/methanol. Also in Anal. Biochem a few years
ago was a method that uses phenol/ether extraction. The nice thing about this method is that
the protein is never actually precipitated, but extracted into the phenolic phase. Therefore,
there is no trouble solubilizing the protein afterwards.
