Steve Dunn (dunnsm at bbsrc.ac.uk) wrote:
: Any ideas how to successfully combine X114 extraction with SDS-PAGE??
: All suggestions gratefully received...
Steve:
I have limited experience with TX114, but did not experience the problem
you described. I followed Bordier's J. Biol. Chem. vol. 256: 1604--1607
paper including equilibration of the detergent and the TX114/sucrose
cushion spins. I didn't see what I wanted to see, but the gel looked ok!
Regards,
Steve Dahl
